Growth stage of F1 generation was recorded into seven stage according to growth phase from one-day old fry, 7-day old fry, 14-day old fry, 28-day old fry, 42-day old fry and 56-day old fry until juvenile. The growth phase was referred to the previous study by Shahjahan et al. (2013).
From day 1 until day 14, newborn guppy was transparent and appear with a tiny colour pigment cell that appears on body surface include caudal fin. From day 28 until day 56, the offspring were an exhibit with a presence of a colour pigment from yellow, red, black and blue on the body surface include caudal fin. However, there are evident shown their colouration on male guppy fry may accumulate with the presence of mitotic activity during the first 3 or 4 weeks (Power & Rao, 1984).
The colour usually occurred by a pigment patterns where it is a predominantly result in the positioning of differently coloured known as chromatophores. It has involved with a many subclasses colour presence in pigment-containing organelles include xanthophores (yellow), erythrophores (red/orange), iridophores (reflective/iridescent), leucophores (white), melanophores (black/brown) and the more rare cyanophores (blue) (Svensson & Sköld, 2011).
Theoretically, the pigment cell might result in long-range patterning mechanisms that include local environmental cues or interactions between neighbouring chromatophores (Kelsh, 2004). So, the colour that presents on the male body may function as attraction or selection by the female which indicate a good gene to their offspring (Houde, 1997).
After day 56, the fry exhibits a marked sexual dimorphism (Khoo et al., 2007) were a different appearance show between male and female in same species that include colour, size and shape.
The importance to enhance the colour of fry guppy were dependently on nutritional content in feed diet. However, early stage like one-day old guppy was given a live feed such as Artemia sp. and Microworm (Panagrellus sp.) until it reaches the size at day 7 where it has been feed with a fine pellet.